Reporter

Part:BBa_K2605009

Designed by: Kaitlin Schaaf   Group: iGEM18_Calgary   (2018-10-09)


CMV with 5' SalI restriction site + mCherry + BGH

Composite part of CMV promoter Part BBa_K2605001 fused to a mCherry reporter gene with BGH terminator (Part BBa_K2605000). This mCherry protein is derived from mushroom coral, Discoma sp. (Shaner et al. 2004).

The part contains a SalI restriction site on the 5' end and a BmtI restriction site on the 3' end to allow for this part to be used within our Multiple-Cloning Site flanked by FRT sites (Part BBa_K2605002).

References

Shaner N.C., Campbell R.E., Steinbach P.A., Giepmans B.N., Palmer A.E., Tsien R.Y. (2004).Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, , 1567-1572

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1556
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 565
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None